AVITI™ Amplicon Sequencing: High-Accuracy, Cost-Efficient NGS

Amplicon sequencing is a targeted approach that allows researchers to focus on specific regions of DNA within a complex sample. By amplifying selected regions and sequencing them at high depth, it can support applications ranging from rare variant detection to microbial community profiling.

There are several ways to approach amplicon sequencing, depending on project requirements. At Source Genomics, we offer Element Biosciences AVITI™ sequencing as a high-accuracy, cost-efficient option for targeted sequencing.

For amplicon sequencing, AVITI™ offers a combination of high accuracy and cost efficiency that can be particularly beneficial for targeted sequencing projects.

Why choose the AVITI™ System for your amplicon sequencing?

The Element Biosciences AVITI™ System combines high sequencing accuracy with features that can benefit amplicon sequencing projects.

Key benefits include:

• Industry-leading accuracy
• Cost-efficient sequencing
• No PCR error propagation
• Negligible index hopping
• Low duplication rates
• Low AT/GC bias

Together, these features can support high-quality sequencing data while helping researchers make efficient use of their sequencing budget.

High accuracy for targeted sequencing

AVITI™ uses Avidity Sequencing with rolling circle amplification (RCA) to deliver high-quality sequencing data.

Source Genomics’ AVITI™ service achieves:

✔ 90% of reads above Q30

✔ 80% of reads above Q40

✔ Up to 70% of reads above Q50 with the UltraQ workflow

For amplicon sequencing projects where accuracy and sensitivity are important, this provides a high-performance option for targeted sequencing.

Cost-efficient amplicon sequencing

Amplicon sequencing focuses sequencing capacity on specific regions rather than an entire genome. This makes it a targeted approach to genomic analysis.

AVITI™ provides a cost-efficient sequencing option, which can be particularly valuable for amplicon projects where high-quality data and sequencing depth are required.

By combining high accuracy with cost efficiency, AVITI™ can help researchers make efficient use of their sequencing budget.

Reliable sequencing data

AVITI™ is designed to support reliable sequencing data through several features that can be particularly useful for targeted sequencing.

Negligible index hopping helps maintain sample identity and data integrity when sequencing multiple samples.

Low duplication rates support efficient use of sequencing data and help maintain representation of the original DNA fragments.

Low AT/GC bias supports balanced coverage across targeted sequences and challenging genomic regions.

What can AVITI™ amplicon sequencing be used for?

Researchers can use amplicon sequencing across a range of genomic applications where specific regions of DNA are the focus.

AVITI™ amplicon sequencing can support applications including:

• Rare variant detection
• Microbial profiling
• Targeted genomic analysis
• Genetic diversity studies
• Variant validation

For projects where sensitivity, accuracy and cost efficiency are important, AVITI™ provides a high-performance option for targeted sequencing.

AVITI™ sequencing at Source Genomics

Source Genomics offers commercial NGS services using the Element Biosciences AVITI™ System from our UK-based laboratory.

As part of our wider sequencing offering, AVITI™ provides another option for researchers looking for precise, cost-efficient targeted sequencing. Our team can help you determine whether AVITI™ is suitable for your amplicon sequencing requirements and discuss your project before you get started.