Delivering unrivalled turnaround times for sequencing using our streamlined SpeedREAD™ data delivery system, providing high-quality results within 12 hours from sample receipt.
Sanger Sequencing Overview
Source Genomics offers market-leading Sanger sequencing services generating up to 1,400 bp of high-quality data per sample. Backed by an ISO-accredited laboratory, our services deliver full traceability and assured data integrity. With flexible sample formats, access to approximately 40 common primers at no cost, and custom primer synthesis options, we support research and development teams across academic, biotech, and pharmaceutical organisations.
Our Sanger sequencing service for plasmid DNA is optimised to generate up to 1,400 bp of clear, dependable sequence data using DNA supplied at 100 ng/ÎĽl. Results are delivered within 12 hours via SpeedREAD. The service supports verification of cloned inserts, mutation checking, and confirmation of promoter fusions or tagged constructs, providing a reliable checkpoint for quality assurance in experimental and production environments.
- Features: Stock and custom primers available; secondary structure treatment for challenging templates; tube and 96-well plate formats; freepost shipping.
- Template Requirements: Plasmid DNA at 100 ng/μl (5µl per reaction); sequencing primers at 3.2 pmol/μl (5µl per reaction).
Plasmid Extractions with Sequencing
Our automated SPRI paramagnetic bead-based extraction service purifies high- and low-copy number plasmids, delivering much faster prep times than manual kits. Submit 1 mL overnight culture or a pea-sized cell pellet. We extract and sequence your samples using primers of your choice, with results delivered within 24 hours.
- Advantages: Fast turnaround; automated high-throughput processing; direct laboratory contact; high customer satisfaction; local UK provider.
- Template Requirements: Cell pellet from 1 mL overnight culture (minimum 15 hours) in 1.5 mL Eppendorf; liquid culture to be incubated overnight; sequencing primers at 3.2 pmol/μl (300µl per plate).
Our Sanger sequencing service for sequencing PCR product has been optimised to generate good quality data if supplied at 10 ng/ÎĽl. The Sequencing data is collected and delivered within 12 hours of sample receipt via our speedREAD service.
Rolling Circle Amplification / Templification
Rapidly generate high-quality DNA templates using plasmid templification and rolling circle amplification (RCA). This approach enables efficient amplification of circular DNA templates, providing abundant material for downstream applications
Colony PCR with Sequencing
Our integrated Colony PCR service combines direct PCR amplification, product purification, and Sanger sequencing into a single workflow. Submit 150 µL glycerol-free overnight culture or extracted genomic DNA. We amplify your target region using your primers, purify products to remove contaminants, and deliver sequencing results within 24 hours to confirm construct presence, size, and orientation.
- Features: Fully integrated workflow; choice of custom or validated stock primers; PCR product purification; enhanced chemistry for GC-rich or complex templates; expert consultation available.
- Template Requirements: 150 µL overnight culture (glycerol-free) in 96-well plate or extracted gDNA; colony PCR primers at 10 pmol/μl (100µl per primer per plate); sequencing primers at 3.2 pmol/μl (300µl per plate).
Key Benefits
Why Choose Sanger Sequencing
While next-generation sequencing enables large-scale discovery, Sanger sequencing remains unmatched for targeted confirmation. It's the method of choice when accuracy, traceability, and regulatory confidence are essential, making it the backbone of sequence verification and QC across research and development and manufacturing pipelines.
Applications include construct and plasmid verification, CRISPR and gene-edit validation, colony screening, quality control of gene therapy products, biomarker validation, and confirmatory sequencing of NGS results.
Optional Add-Ons
- Secondary Structure Treatment: For G-C-rich sequences, hairpin-containing or repetitive regions, our dGTP chemistry disrupts secondary structure formation, improving read quality.
- Custom Primer Synthesis: Beyond our 40 stock primers, we offer custom synthesis for specialised targets.
How to Order
Register at our Sequencing Portal and select your template type (Plasmid DNA, PCR Products, Colony PCR, or Plasmid Extractions). Enter sample and primer details, complete analysis settings, and submit. Ship samples using Freepost labels or drop boxes—we'll process immediately and deliver results within 12-24 hours by email.
Contact us today and one of our skilled account managers will be in touch with a free consultation including further information and pricing details.
Rapid Sanger Sequencing
Delivering unrivalled turnaround times for sequencing using our streamlined SpeedREAD™ data delivery system. Providing high-quality data retrieval within 12 hours from sample receipt.