Rolling Circle Amplification (RCA) Now Available

At Source Genomics, we are always looking for ways to improve the accuracy and efficiency of our sequencing services. That’s why we are excited to introduce Rolling Circle Amplification (RCA) as a pre-treatment option for our Nanopore Plasmid Sequencing service. RCA is a powerful molecular technique that enhances sequencing accuracy and yield, making it an excellent choice for researchers who require high-quality plasmid analysis.

What is Rolling Circle Amplification (RCA)?

Rolling Circle Amplification (RCA) is an isothermal amplification method that selectively amplifies circular DNA molecules, such as plasmids, with high fidelity. This process generates long DNA concatemers, significantly improving sequencing quality and read length while reducing potential sequencing errors.

Why Choose RCA for Your Plasmid Sequencing?

By incorporating RCA into your Nanopore Plasmid Sequencing workflow, you can benefit from:

• Enhanced Accuracy – RCA amplifies the plasmid DNA, improving sequencing reliability and reducing potential errors.
• Longer Read Lengths – Increased sequence coverage enables more comprehensive plasmid characterisation.
• Higher Yield – Optimised sample preparation leads to improved sequencing depth and output.
• Seamless Ordering Process – RCA is available as an add-on in our ordering portal for Nanopore 30, Nanopore 150, and Nanopore 300.

Fast Turnaround Time

We understand that time is crucial in research, which is why our enhanced sequencing workflow delivers results in less than 12 hours from sample receipt!* This rapid turnaround ensures you get the data you need when you need it.

Sample Requirements

To take advantage of RCA, simply provide us with a small volume of overnight culture (around 50 μl). There’s no need for plasmid DNA extraction – we can sequence the entire plasmid directly from the overnight culture, streamlining your workflow even further.

Get Started Today

If you’re looking to optimise your plasmid sequencing results, RCA is the perfect solution. Contact us today to learn more about how this powerful add-on can enhance your research.

*Turnaround time is dependent on sample submission time and laboratory workflow.