The Colony PCR service is a PCR fragment amplification service, generating PCR products suitable for Sanger Sequencing. The service requires a direct PCR from a 96 well plate of Liquid Culture, processed with 2 primers that amplify the region of interest. We then purify the 96 well plate of PCR products and Sequence the fragments with a Sequencing primer(s). The Sequencing data is collected and delivered within 24 hours of sample receipt.
A free consultation is available to discover more about the Sequencing services we can offer you.
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Sequencing Portal Video Guides
For a list of user guide videos that walks you through how to place orders on the sequencing portal click here.
How Does it Work?
Register for an online account here.
Creating a Sanger Sequencing Order
Begin placing your sequencing order here.
Select the appropriate ‘Template Type’. The screen will adjust so you can provide details of the template and confirm that you have read and understood the sample requirements.
Prepare Your Samples
Enter details of the sample submission format including tubes, plates and orientation of the plate.
Primer Synthesis (optional add-on)
Source BioScience stocks a collection of 40 common primers from which you can choose (free of charge) for your Sanger Sequencing. If, however, you require a primer/s that we do not stock, you might like to make use of our Primer Synthesis option.
Secondary Structure Resolution (optional add-on)
When sequencing templates which are G-C-rich, contain hairpin structures, repetitive regions or troublesome sequences, we recommend including our Secondary Structure Treatment Solution (dGTP chemistry) with your order.
This optional add-on aids the sequencing process by disrupting the production of secondary structures, as well as aiding the initiation of the sequencing process, to yield higher quality reads.
150ul of Overnight Culture – glycerol free where possible, supplied in a 96 well plate.
A forward and reverse colony primer is required to amplify region of interest. Colony primers to be sent at a concentration of 10pmol/ul in a volume of 100ul per primer, per plate.
Sequencing primers to be sent at a concentration of 3.2pmol/ul in a volume of 300ul per plate.
Complete the Analysis settings required for this order. Each question mark will open new page with a detailed explanation of the particular option. You can also add additional recipients for the data.
This information can be added individually or added as an Excel template. If adding the sample and primer details manually, the list of free stock primers will be available from our drop down list. Samples which have been correctly recorded will appear in the Job Summary. Once the details are recorded, click ‘Submit Application’.
Sending Your Samples
Place your samples in a secure package marked with the order number, and either deposit them in your Source Genomics drop box for collection, or use our FREEPOST labels to send your samples to us by post. Samples will be processed as soon as they arrive at a Source Genomics lab, and your data will be with you within 24 hours of sample receipt.
Your samples will be processed as soon as they arrive at Source Genomics, and your data will be emailed within 24 hours of sample receipt.
Note: You are now able to purchase eVouchers to cover all types of pre-treatment and Sequencing reactions enabling you to place your orders more simply.
Established as a market-leading Sanger Sequencing service provider, we deliver rapid turnaround times for sequencing using our streamlined SpeedREAD™ data delivery system, to the highest quality.