As plasmid designs become more intricate, so does the need for complete, high-resolution sequencing. Source Genomics delivers full plasmid coverage using long-read nanopore sequencing to reveal features short-read platforms often miss. With rapid turnaround, minimal preparation, and clear, ready-to-use data, our whole plasmid nanopore sequencing services gives researchers the confidence to move forward with their experiments and production workflows.
Rapid Turnaround Times
Delivering unrivalled turnaround times for Whole Plasmid Nanopore Sequencing using our streamlined SpeedREAD™ data delivery system. Providing high-quality data retrieval within 12 hours* from sample receipt.
*May vary depending on your exact requirements. Contact us in case of any questions. 10 hours or less from sample receipt to results in the vast majority of cases.
Whole Plasmid Nanopore Sequencing Services
We deliver a flexible range of sequencing solutions tailored to different plasmid sizes and project goals. All services are powered by Oxford Nanopore technology and include expert guidance from sample preparation through to data delivery.
Amplicon
Verifies specific plasmid regions when full-length analysis is not required.
Nanopore30
Suitable for plasmids up to 30 kilobases, commonly used in cloning and standard vector systems.
Nanopore150
Designed for constructs up to 150 kilobases, including expression vectors and gene therapy templates.
Nanopore300
Handles large plasmids and BACs up to 300 kilobases, supporting synthetic biology and advanced genomic work.
Multiplexed barcoding
Enables up to 96 plasmids to be sequenced simultaneously, reducing turnaround and cost per sample.
SpeedREAD™ delivery
Ensures you receive your data typically around 12 hours of sample arrival.
These options are designed for reliability and scalability, whether you're sequencing a single construct or coordinating a high-throughput screening project.
Rolling Circle Amplification (RCA)
To increase sequencing quality and consistency, particularly in low-yield or structurally complex plasmid samples, we provide RCA as an optional enhancement. RCA is a highly efficient method that selectively amplifies circular DNA to produce clean, high-coverage input for nanopore sequencing.
By using RCA, you can:
Adding RCA helps unlock more detail from challenging plasmids, supporting a higher standard of sequence clarity and reliability. It’s particularly powerful in high-detail workflows like Nanopore30 and can be integrated where it adds the most value across our service options.
The best part? Results in less than 12 hours from sample receipt!**
Requirements: A small volume of overnight culture (around 50 ÎĽl).
Note: Plasmid DNA does not need to be extracted—we can sequence the entire plasmid directly from the overnight culture.
**May vary depending on your exact requirements. Contact us in case of any questions. 12 hours or less from sample receipt to results in the vast majority of cases.
Benefits
Choosing Source Genomics for whole plasmid nanopore sequencing gives you:
A free consultation is available to discover more about the Sequencing services we can offer you.
Contact us in case of any questions.
How To Order
For optimal results we recommend using a Qubit for DNA quantification, as it can distinguish dsDNA, even in extremely small amounts or in the presence of contaminants.
NanoporeAmplicon
15ul @30ng/ÎĽl per sample
Nanopore30
15ul @30ng/ÎĽl per sample
Nanopore150
30ul @30ng/ÎĽl per sample
Nanopore300
50ul @30ng/ÎĽl per sample
Rolling Circle Amplification (RCA)
A small volume of overnight culture (around 50 ÎĽl).
Note: Plasmid DNA does not need to be extracted—we can sequence the entire plasmid directly from the overnight culture.
Sending Your Samples
Place your samples in a secure package marked with the order number, and either deposit them in your Source Genomics drop box for collection, or use our FREEPOST labels to send your samples to us by post.
Receive Data
Your samples will be processed as soon as they arrive at Source Genomics, and your data will be emailed within 12 hours* of sample receipt.
May vary depending on your exact requirements. Contact us in case of any questions. 12 hours or less from sample receipt to results in the vast majority of cases.
Let's Move Your Project Forward
Source Genomics makes plasmid sequencing straightforward, reliable, and ready to meet the pace of your work. If you're looking for speed, clarity, and confidence in every result, we’re here to help. Our team delivers not just data, but the clarity to make confident decisions at every step of your project, combining advanced technology with practical expertise to support your goals from beginning to end.
Contact us today and one of our skilled account managers will be in touch with a free consultation including further information and pricing details.