Sanger Sequencing FAQs

Source BioScience offers a range of single read and 96 well plate eVouchers. Click on the following link to find out more and order your Sanger Sequencing eVouchers.

Each reaction that is ordered on the website requires one eVoucher to cover the cost of the reaction. When you get to the ‘your basket’ page on the order form simply input the number of eVouchers you are using on the job and click update cart in the bottom right-hand corner. This will subtract the cost of the eVouchers from the order. eVouchers are automatically deducted from your account. If you are submitting a full plate for sequencing then 96 single read eVouchers or 1x 96 well plate eVouchers will be required as payment.

Yes, you are now able to use specific e-vouchers for all Sanger pre-treatments and types of order. The full range of e-vouchers can be purchased here.

Our eVouchers page allows you to buy vouchers in single reaction or plate format for all the pre-treatments. These are purchased and added to your account, you are able to transfer these to other users in your organisation as well if you wanted one main purchaser. This means you no longer need a Purchase Order number to cover the extra.

The sample requirements for Sequencing can be found in the following link DNA and Primer Sample Requirements.

Yes, we now order stand-alone primers using this link. Primers can be purchased using e-vouchers or via a Purchase Order number.

Primers that are ordered through the website are stored for a year and can be selected from the drop-down box on the reaction details page.

You can select this as an option when placing your order. Select the most recent order/s as required from your available options and then continue to place your order as usual. Your previous samples/primers are available in the drop-down menu when you are selecting your reaction specifics for you to choose as required.

Yes, all primers we process need to be sent to us in separate tubes so that we can prepare the Master Mixes with the correct ratio of reagents and primer.

Yes, although we require primers sent at different concentrations for the Colony PCR and Sequencing. Our primer requirements can be found here.

You can check what primers Source BioScience stock by clicking here.

If you are unable to generate the volume and concentration of DNA we require then please contact the laboratory or our sales team who can advise you  whether it is worth sending the samples. The sequencing reactions can be optimised to work with stronger/weaker DNA.

In Sanger Sequencing we offer the following reaction types and pre-treatments, all are available to be purchased as eVouchers for your convenience.

Plasmid DNA
Standard processing of a Plasmid sample through Sanger Sequencing.

Template Amplification
A pre-treatment for Plasmid samples only, this service involves a 12hour incubation period to increase DNA concentration and remove any inhibitors.

PCR Product
Standard processing of a PCR sample through Sanger Sequencing.

PCR Clean-up
A pre-treatment for PCR samples only. The submitted samples are purified prior to processing to remove any excess dyes/salts or other inhibitors that are present that can interfere with the reaction.

Premixed
This service is for processing prepared samples where they have already been combined with the primer in the required quantities and concentrations. Please note no repeats are able to be processed on these reactions as we are unable to optimise the reaction conditions.

Sanger Run Only
This service is where customers supply a purified sequencing reaction that is ready for processing on the sequencer. Their DNA and primer have been PCR cycled with a dye terminator kit and subsequent purification has been processed.

Sanger Run Only Clean-up
This is a pre-treatment service for Sanger Run Only samples where the DNA and primer have been PCR cycled with the dye terminator kit and the reactions simply require the final purification prior to running on the sequencer.

Plasmid Extraction
This service is for when we are required to extract the plasmid from a cell pellet or liquid culture that is submitted prior to sequencing.

Colony PCR
This is a fragment amplification service where we generate a PCR product using submitted primers prior to processing purifying and sequencing the sample.

Nanopore30
This service is for sequencing full plasmids without the use of primers.

Genotyping
Our Microsatellite Genotyping service utilises the 3730xl DNA Analysers to run pre-prepared Genotyping plates through the capillary resulting in super-fast data delivery. We stock a small range of Size Standards and DyeSets for customers to select during the ordering process.

Customer submitted samples and primers are stored for 3 weeks and are available to re-use within this timeframe. You can select to reuse them when placing the order, then selected the previous order number. If the order number isn’t displayed, the samples/primers will have been discarded. If you would like to store a primer for a longer period then we recommend you order it through our Primer Synthesis service where it is stored for you for 1 year.