Primer Synthesis

Primers currently in stock

PRIMER (alphabetically) SEQUENCE Β  Β  LENGTH % G+C Tm (Β°C)
30nt_M13R AGC GGA TAA CAA TTT CAC ACA GGA 24 42 59
ACYC Duet Up1 GGA TCT CGA CGC TCT CCC T 19 63 55
BGH Reverse TAG AAG GCA CAG TCG AGG 18 56 50
C.elegansRNAi F GTT TTC CCA GTC ACG ACG TT 20 50 64
CMVF_pCDNA3 CAA CGG GAC TTT CCA AAA TG 20 45 55
Duet Down1 GAT TAT GCG GCC GTG TAC AA 20 50 52
EGFP C F CAT GGT CCT GCT GGA GTT CGT G 22 59 59
EGFP C R GTT CAG GGG GAG GTG TG 17 65 62
EGFP Nrev CGT CGC CGT CCA GCT CGA CCA G 22 73 64
KS CCT CGA GGT CGA CGG TAT CG 20 65 58
M13R CAG GAA ACA GCT ATG ACC 18 50 48
M13F TGT AAA ACG ACG GCC AGT 18 50 48
PBADF ATG CCA TAG CAT TTT TAT CC 20 35 58
PBADR GAT TTA ATC TGT ATC AGG 18 33 45
pCMV forward GAG CTC GTT TAG TGA ACC GTC 21 52 54
pCMV reverse CAA GGC CAG GAG AGG CAC TG 20 65 58
PDONR201 3PRIME CAT CAG AGA TTT TGA GAC AC 20 40 54
PDONR201 5PRIME GCA GTT CCC TAC TCT CGC 18 61 60
PDONR201 Forward TCG CGT TAA CGC TAG CAT GGA TCT C 25 52 72
PDONR201 Reverse GTA ACA TCA GAG ATT TTG AGA CAC 24 37 58
pEXPRESS_F TAT GGC TAG CAT GAC TGG t 19 47 49
PGEX 3' CCG GGA GCT GCA TGT GTC AGA GG 23 65 62
PGEX 5' GGG CTG GCA AGC CAC GTT TGG TG 23 65 62
malEforward GGT CGT CAG ACT GTC GAT GAA GCC 24 58 61
PME18SF CTT CTG CTC TAA AAG CTG CG 20 50 62
PME18SR GCA CCT GCA GCT CGA GCA CA 20 65 73
POLYA AAA AAA AAA AAA AAA AAA AAA AAA B 25 0 57
POLYT TTT TTT TTT TTT TTT TTT TTT TTT V 25 0 58
PQE30 forward CCC GAA AAG TGC CAC CTG 18 61 53
PQE30 reverse GTT CTG AGG TCA TTA CTG G 19 47 49
pTargeT reverse TTA CGC CAA GTT ATT TAG GTG ACA 24 38 63
pTrcHis_F GAG GTA TAT ATA TTA ATG TAT CG 23 29 45
SK CGG CCG CTC TAG AAC TAG TGG ATC 24 58 61
SP6 promoter ATT TAG GTG ACA CTA TAG 18 33 41
T3 ATT AAC CCT CAC TAA AG 17 35 40
T7 promoter (F) TAA TAC GAC TCA CTA TAG GG 20 40 48
T7 terminator (R) GCT AGT TAT TGC TCA GCG G 19 53 51
pCEP-Forward AGA GCT CGT TTA GTG AAC CG 20 50 52
pCEP-Reverse GTG GTT TGT CCA AAC TCA TC 20 45 50
pEF1a fwd ATT TGC CCT TTT TGA GTT TGG 21 38 5

Disclaimer

Source BioScience accepts no responsibility for failed sequencing reads when customers select any of the above primers but have not checked to see if these primer sequences bind to their template. The above primers do not necessarily have the same content as primers of the same or similar name that can be found elsewhere.

Sample storage

We keep your templates and primers up to 3 weeks, unless instructed otherwise. If you would like any of your materials kept for longer, then simply let us know and we will move them into longer term storage. Materials in long term storage that have not been used for a period of 3 months will be disposed of, unless you instruct us otherwise.

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