Primers currently in stock
| PRIMER (alphabetically) | SEQUENCE | ย ย LENGTH | % G+C | Tm (ยฐC) |
| 30nt_M13R | AGC GGA TAA CAA TTT CAC ACA GGA | 24 | 42 | 59 |
| ACYC Duet Up1 | GGA TCT CGA CGC TCT CCC T | 19 | 63 | 55 |
| BGH Reverse | TAG AAG GCA CAG TCG AGG | 18 | 56 | 50 |
| C.elegansRNAi F | GTT TTC CCA GTC ACG ACG TT | 20 | 50 | 64 |
| CMVF_pCDNA3 | CAA CGG GAC TTT CCA AAA TG | 20 | 45 | 55 |
| Duet Down1 | GAT TAT GCG GCC GTG TAC AA | 20 | 50 | 52 |
| EGFP C F | CAT GGT CCT GCT GGA GTT CGT G | 22 | 59 | 59 |
| EGFP C R | GTT CAG GGG GAG GTG TG | 17 | 65 | 62 |
| EGFP Nrev | CGT CGC CGT CCA GCT CGA CCA G | 22 | 73 | 64 |
| KS | CCT CGA GGT CGA CGG TAT CG | 20 | 65 | 58 |
| M13R | CAG GAA ACA GCT ATG ACC | 18 | 50 | 48 |
| M13F | TGT AAA ACG ACG GCC AGT | 18 | 50 | 48 |
| PBADF | ATG CCA TAG CAT TTT TAT CC | 20 | 35 | 58 |
| PBADR | GAT TTA ATC TGT ATC AGG | 18 | 33 | 45 |
| pCMV forward | GAG CTC GTT TAG TGA ACC GTC | 21 | 52 | 54 |
| pCMV reverse | CAA GGC CAG GAG AGG CAC TG | 20 | 65 | 58 |
| PDONR201 3PRIME | CAT CAG AGA TTT TGA GAC AC | 20 | 40 | 54 |
| PDONR201 5PRIME | GCA GTT CCC TAC TCT CGC | 18 | 61 | 60 |
| PDONR201 Forward | TCG CGT TAA CGC TAG CAT GGA TCT C | 25 | 52 | 72 |
| PDONR201 Reverse | GTA ACA TCA GAG ATT TTG AGA CAC | 24 | 37 | 58 |
| pEXPRESS_F | TAT GGC TAG CAT GAC TGG t | 19 | 47 | 49 |
| PGEX 3' | CCG GGA GCT GCA TGT GTC AGA GG | 23 | 65 | 62 |
| PGEX 5' | GGG CTG GCA AGC CAC GTT TGG TG | 23 | 65 | 62 |
| malEforward | GGT CGT CAG ACT GTC GAT GAA GCC | 24 | 58 | 61 |
| PME18SF | CTT CTG CTC TAA AAG CTG CG | 20 | 50 | 62 |
| PME18SR | GCA CCT GCA GCT CGA GCA CA | 20 | 65 | 73 |
| POLYA | AAA AAA AAA AAA AAA AAA AAA AAA B | 25 | 0 | 57 |
| POLYT | TTT TTT TTT TTT TTT TTT TTT TTT V | 25 | 0 | 58 |
| PQE30 forward | CCC GAA AAG TGC CAC CTG | 18 | 61 | 53 |
| PQE30 reverse | GTT CTG AGG TCA TTA CTG G | 19 | 47 | 49 |
| pTargeT reverse | TTA CGC CAA GTT ATT TAG GTG ACA | 24 | 38 | 63 |
| pTrcHis_F | GAG GTA TAT ATA TTA ATG TAT CG | 23 | 29 | 45 |
| SK | CGG CCG CTC TAG AAC TAG TGG ATC | 24 | 58 | 61 |
| SP6 promoter | ATT TAG GTG ACA CTA TAG | 18 | 33 | 41 |
| T3 | ATT AAC CCT CAC TAA AG | 17 | 35 | 40 |
| T7 promoter (F) | TAA TAC GAC TCA CTA TAG GG | 20 | 40 | 48 |
| T7 terminator (R) | GCT AGT TAT TGC TCA GCG G | 19 | 53 | 51 |
| pCEP-Forward | AGA GCT CGT TTA GTG AAC CG | 20 | 50 | 52 |
| pCEP-Reverse | GTG GTT TGT CCA AAC TCA TC | 20 | 45 | 50 |
| pEF1a fwd | ATT TGC CCT TTT TGA GTT TGG | 21 | 38 | 5 |
Disclaimer
Source BioScience accepts no responsibility for failed sequencing reads when customers select any of the above primers but have not checked to see if these primer sequences bind to their template. The above primers do not necessarily have the same content as primers of the same or similar name that can be found elsewhere.
Sample storage
We keep your templates and primers up to 3 weeks, unless instructed otherwise. If you would like any of your materials kept for longer, then simply let us know and we will move them into longer term storage. Materials in long term storage that have not been used for a period of 3 months will be disposed of, unless you instruct us otherwise.
Contact us today and one of our skilled account managers will be in touch with a free consultation including further information and pricing details.
Rapid Sequencing
Delivering unrivalled turnaround times for sequencing using our streamlined SpeedREADโข data delivery system. Providing high-quality data retrieval within 12 hours from sample receipt.