Source BioScience delivers fast, reliable Sanger sequencing through our proprietary SpeedREAD™ system, providing results within 12 hours. Specialising in plasmid and PCR product sequencing, we generate up to 1,400 bp of high-quality data per sample, supported by ISO-accredited laboratories that ensure full traceability and data integrity.
Sanger DNA Sequencing
Whether you’re driving biopharmaceutical innovation, validating cloned constructs, or confirming precise edits in your research pipeline, Sanger DNA sequencing remains the gold standard for sequence verification. Known for its unmatched accuracy, Sanger sequencing enables fully traceable highly targeted analyses of specific genes or DNA fragments with precision and confidence.
Source BioScience delivers fast, reliable Sanger sequencing through our proprietary SpeedREAD™ system, providing results within 12 hours. Specialising in plasmid and PCR product sequencing, we generate up to 1,400 bp of high-quality data per sample, supported by ISO-accredited laboratories that ensure full traceability and data integrity.
With flexible sample formats, free access to 40 common primers, and custom primer synthesis options, our service adapts to the needs of academic, biotech, and pharmaceutical R&D teams alike.
Applications Across Research & Development
Our Sanger sequencing supports a wide range of critical research and development activities, including:
- Construct and plasmid verification for cloning and expression vectors
- CRISPR and gene-edit validation, confirming on-target edits and mutation integrity
- Quality control and release testing of gene therapy or recombinant DNA products
- Pharmacogenetic marker and biomarker validation in preclinical studies
- Confirmatory sequencing of NGS results for regulatory submissions
Trusted by leading biopharma, biotech, and academic institutions, our Sanger sequencing services ensure every sequence you rely on is accurate, reproducible, and regulatory-ready.
A free consultation is available to discover more about the Sequencing services we can offer you.
How Sanger Sequencing Works
Sanger sequencing operates through chain-termination chemistry, where fluorescently labeled dideoxynucleotides (ddNTPs) are selectively incorporated during DNA replication. This produces DNA fragments of varying lengths, which are then separated via capillary electrophoresis. The sequence is determined by reading the fluorescently tagged terminal bases in order of fragment size.
Our optimised workflows combine:
- Cycle sequencing reactions for high signal clarity
- Automated base calling and Phred quality scoring for >99.9% accuracy
- Expert data review and free repeat sequencing for any failed reads
These quality assurances ensure consistent, publication and submission ready data.
Sanger vs. NGS — The Right Tool for Precision
While next-generation sequencing (NGS) enables large-scale discovery, Sanger sequencing remains unmatched for targeted confirmation. It is the method of choice when accuracy, traceability, and regulatory confidence are essential; making it the backbone of sequence verification and QC across R&D and manufacturing pipelines.
Your Global Sanger Sequencing Partner
Our service combines speed, reliability, and compliance to support researchers worldwide:
An intuitive online portal streamlines submissions and results retrieval, with video guides to walk you through every step. Interested in how our Rapid sequencing service might work for you?
Sequencing Portal Video Guides
Click here for a list of user guide videos that walk you through placing orders on the sequencing portal.
Primers Currently In Stock
For an existing list of primers in stock, please click here.
Our experienced scientists are available for free consultations about your unique research challenges: project planning, chromatogram interpretation, troubleshooting difficult templates, and more. Contact our team to discuss your project needs and receive tailored guidance on optimising your sequencing strategy.
Rapid Sanger Sequencing
Delivering unrivalled turnaround times for sequencing using our streamlined SpeedREAD™ data delivery system. Providing high-quality data retrieval within 12 hours from sample receipt.