Our Sanger Sequencing service has been optimised to generate up to 1400bp of good quality data using plasmid DNA supplied at 100 ng/Ī¼l. The Sequencing data is collected and delivered within 12 hours of sample receipt via our speedREAD service.
A free consultation is available to discover more about the Sequencing services we can offer you.
High-Accuracy Sequencing for Plasmid DNA
Plasmid DNA is a core component of countless lab workflows. As a circular genetic element, plasmids enable gene expression, drive site-directed mutagenesis, support recombinant vector construction, and underpin the development of many DNA-based therapeutics. Before any of these applications can progress, the sequence needs to be verified. Thatās why researchers turn to Sanger sequencing. It offers the clarity needed to confirm a construct, validate a mutation, or assess a junction. With fast delivery and uncompromising precision, Source Genomics makes this essential step seamless.
Sanger Sequencing Services
Our Sanger sequencing services are purpose-built for plasmid DNA, combining speed, flexibility, and high data quality. By utilising our services, you can:
Each step of the service is streamlined through our online portal. Experienced sequencing specialists are also available to help handle the process from start to finish.
The Sanger Sequencing Process
Sequencing at Source Genomics follows a refined workflow designed for accuracy and consistency when working with plasmid DNA.
- Sample Preparation: Upon receipt, plasmid DNA samples are prepared using protocols that ensure optimal purity and concentration.
- Primer Annealing: Selected primers, either from our stock collection or custom synthesised, are annealed to the DNA template to begin the sequencing reaction.
- Chain Termination Reaction: Specialised reagents produce DNA fragments of varying lengths through the incorporation of chain-terminating nucleotides.
- Capillary Electrophoresis: The resulting fragments are separated by size, enabling precise sequence determination.
- Data Analysis and Delivery: Chromatograms are reviewed for quality before sequencing data is securely delivered to you.
This approach is ideal for verifying cloned inserts, checking for mutations, and confirming promoter fusions or tagged constructs. Moreover, it provides a reliable checkpoint for quality assurance in both experimental and production environments.
Your Global Rapid Sequencing Partner
Quality Service. Quality Data. Quality People.
Sequencing Portal Video Guides
For a list of user guide videos that walks you through how to place orders on the sequencing portal click here.
How To Order
Place Order
Registration
Register for an online accountĀ here.
Creating a Sanger Sequencing Order
Begin placing your sequencing orderĀ here.
Template Type
Select the appropriate āTemplate Typeā. The screen will adjust so you can provide details of the template and confirm that you have read and understood theĀ sample requirements.
Prepare Your Samples
Enter details of the sample submission format including tubes, plates and orientation of the plate.
Primer Synthesis (optional add-on)
Source BioScience stocks a collection ofĀ 40 common primersĀ from which you can choose (free of charge) for your Sanger Sequencing. If, however, you require a primer/s that we do not stock, you might like to make use of our Primer Synthesis option.
Secondary Structure Resolution (optional add-on)
When sequencing templates which are G-C-rich, contain hairpin structures, repetitive regions or troublesome sequences, we recommend including our Secondary Structure Treatment Solution (dGTP chemistry) with your order.
This optional add-on aids the sequencing process by disrupting the production of secondary structures, as well as aiding the initiation of the sequencing process, to yield higher quality reads.
Template Requirements
Plasmid DNA to be supplied at a concentration of 100 ng/Ī¼l in a volume of 5ul per reaction.
Sequencing primers to be sent at a concentration of 3.2 pmol/Ī¼l in a volume of 5 Ī¼l per reaction.
Analysis Settings
Complete the Analysis settings required for this order. Each question mark will open new page with a detailed explanation of the particular option. You can also add additional recipients for the data.
Reaction Details
This information can be added individually or added as an Excel template. If adding the sample and primer details manually, the list ofĀ free stock primersĀ will be available from our drop down list. Samples which have been correctly recorded will appear in the Job Summary. Once the details are recorded, click āSubmit Applicationā.
Sending Your Samples
Place your samples in a secure package marked with the order number, and either deposit them in your SourceĀ GenomicsĀ drop box for collection, or use ourĀ FREEPOST labelsĀ to send your samples to us by post. Samples will be processed as soon as they arrive at a Source Genomics laboratory, and your data will be with you within 12 hours of sample receipt.
Receive Data
Your samples will be processed as soon as they arrive at SourceĀ Genomics, and your data will beĀ emailedĀ within 12 hours of sample receipt.
Note: You are now able to purchase eVouchers to cover all types of pre-treatment and Sequencing reactions enabling you to place your orders more simply.
Benefits
Confidence in every read begins with a service built for precision and performance. Our plasmid DNA sequencing offers:
These features work together to accelerate your research, minimise uncertainty, and let you focus fully on your experimental goals.
Benefits
Source Genomics provides the speed, quality, and clarity needed to move your plasmid-based work forward without delays or compromise. Our Sanger sequencing service is built for the demands of modern molecular biology and supported by people who understand it. Send us your samples and weāll take care of the rest. Contact us today to get started.
Contact us today and one of our skilled account managers will be in touch with a free consultation including further information and pricing details.
Rapid Sequencing
Delivering unrivalled turnaround times for sequencing using our streamlined SpeedREADā¢ data delivery system. Providing high-quality data retrieval within 12 hours from sample receipt.